Near-infrared fluorescence imaging showed that the restrained expression of ABCG2 was associated with a slight increase of fluorescence signal in HCC cells incubated with MHI-148 for 60 minutes, but more remarkably, the brightness lasted longer than control (The maximum fluorescence intensity at 60 minutes respectively is 140 ± 8 and 110 ± 4 a.u). This evidence concerns the gene ABCG2 and hepatocellular carcinoma.