To directly investigate the functional role of OATP2B1 in the uptake of MHI-148 dye into HCC cells, cells with the relatively low or high expression of OATP2B1 were selected for the establishment of cell models with knockdown or forced expression of OATP2B1, respectively (Supplementary Figure 3C). The gene discussed is SLCO2B1; the disease is hepatocellular carcinoma.