Overall, our analysis suggests that the low expression of NCAM1 in Mix_Sub subtype patients is likely caused by the large number of copy number deletions, which restrict its binding to FGFR1, resulting in the inability of various downstream signaling pathways to be activated, which in turn led to tumor deterioration and ultimately to poorer survival outcomes for Mix_Sub subtype patients. The gene discussed is FGFR1; the disease is neoplasm.