In conclusion, PBM with 610 nm LED showed anti-rheumatic effects and affected inhibition in cell proliferation, migration, and invasion in RA-FLSs, and in paw thickness, autoantibodies, synovial inflammation, hyperplasia, cartilage damage, pro-inflammatory cytokines, ROS, pyroptosis signals, and TLR4-mediated NF-κB activation in mouse models of CIA. Here, TLR4 is linked to rheumatoid arthritis.