Second, we observed that the protective effect of IFNβ on lessening delayed tPA-aggravated BBB in ischemic stroke was partly reversed in MCAO mice with MG-specific IFNAR1 knockdown, suggesting that IFNβ-mediated modulation of MG phenotypes plays a role in lessening delayed tPA-aggravated BBB disruption in ischemic stroke. Here, IFNB1 is linked to ischemic stroke.