Collectively, our results demonstrate that IFNβ suppresses inflammatory cytokine expression and promotes anti-inflammatory molecule expression in TNFα+tPA-treated MG in vitro, and that confirms our in vivo observation of IFNβ-mediated suppression of inflammatory MG and promotion of anti-inflammatory MG in tPA-treated MCAO mice. This evidence concerns the gene IFNB1 and myasthenia gravis.