At 1 day after tMCAO, our enrichment analysis and MCODE analysis of sc_S4 and sc_S5 cells representing Itgb2+ on day 1 after tMCAO showed that Itgb2+ microglia clusters representing the early stroke phase were undergoing rapid proliferation and differentiation (26, 27), and intense energy metabolism (28). The gene discussed is ITGB2; the disease is Stroke.