In this series, comparing the secretomes of expanded PIT with that of cultured tumor and MPE CFF, we can infer that activation with CD3/CD28 plus IL-2 drives a variety of myeloid chemoattractants, TNFα (which together with IFNγ drives M1 polarization), matrix metalloproteases, effector cytokines, and a host of T-cell growth and differentiation factors. This evidence concerns the gene CD28 and neoplasm.