As shown in Figure 3C-3E, the restored expression of HPRT1 and IMPDH2 mainly rescued the functional defects of tumor cells lacking AFF4, such as cell proliferation (Figure 3C), colony formation (Figure 3D) and cell cycle progression (Figure 3E) to varying degrees, robustly indicating HPRT1 and IMPDH2 are two major downstream targets of AFF4 and the function of AFF4 in PDAC cells mainly depends on driving the expression of HPRT1 and IMPDH2 genes. Here, HPRT1 is linked to neoplasm.