AFF4 and neoplasm: Because the better consistence of AFF4 expression in tumor and normal with survive plot existed in PDAC, we next focused on AFF4 and detected AFF4 protein levels in eight PDAC cell lines, and among these cell lines, AsPC-1 and BxPC-3 expressed higher levels of AFF4 than other cell lines, while CAPAN-2, CFPAC-1 and SW1990 expressed the lowest protein levels of AFF4 (Figure 1C).