To characterize the potential effects of PCDH7 on colorectal cancer, we successfully induced the expression of PCDH7 in SW480, HT29, HCT116, LS411N, and SW620 cells using an overexpression plasmid, while decreasing PCDH7 expression in DLD1 and RKO cells by transfection with a siRNA against PCDH7 (Figure 1B). Here, PCDH7 is linked to colorectal cancer.