HMGB1 and ischemia: (2015) performed specific HMGB1 staining in ischemia‐induced necrotic neurons and astrocytes by using hmgb1 antibody and observed that the cytoplasmic HMGB1 staining was almost uniform in the necrotic neurons, whereas it was granular in the necrotic astrocytes, which indicated that the posttranslational hmgb1 modifications in ischemia‐induced neurons, glial cells, and the secretory pathway may vary.