To understand if kinase activity to phosphorylate UBAP2L is necessary for CRC cell growth, we mutated the UBAP2L Ser 445/454 and Ser 467/477 sites in pairs and expressed mutated Ser 445/454 (A) and Ser 467/477 (A) UBAP2L at levels comparable with those of wild-type UBAP2L in PCK1-OE CRC cells. Here, PCK1 is linked to colorectal carcinoma.