Then, we explored the expression of Rad54l2 in Xp11.2 tRCC and HK-2 cells after estrogen treatment with RNA-sequencing and bioinformatic analysis to study the role of estrogen in Xp11.2 tRCC. This evidence concerns the gene RAD54L2 and renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.