GAEA analysis found that after HK-2 cell treatment with estrogen, Rad54l2-associated differentially expressed genes were also enriched in cancer pathway, including small cell lung cancer, pancreatic cancer, colorectal cancer, non-small cell lung cancer, glioma, acute myeloid leukemia, chromic myeloid leukemia, basal cell carcinoma, melanogenesis, melanoma (Fig. 3A–K), as well as for RCC (Fig. 3L), in addition, endometrial cancer and prostate cancer were also significantly enriched (Fig. 3M,N), which partial caused by hormone in oncogenesis. The gene discussed is RAD54L2; the disease is melanoma.