We then expressed Vps1 variants for several of these pathologies, including microcytic anemia (I277G), Charcot-Marie-Tooth disorder (G397R), centronuclear myopathy (E407K, I422G, and K506W), hereditary spastic paraplegia (R684W), exercise-induced collapse in Labradors (R298L), and a candidate mutation for epilepsy in humans (A447T) in vps1Δ cells to examine autophagic flux using again the Pho8Δ60 assay (Fig. 6A and Table 2). This evidence concerns the gene DNM1L and autosomal dominant centronuclear myopathy.