DNM1L and hereditary spastic paraplegia: We then expressed Vps1 variants for several of these pathologies, including microcytic anemia (I277G), Charcot-Marie-Tooth disorder (G397R), centronuclear myopathy (E407K, I422G, and K506W), hereditary spastic paraplegia (R684W), exercise-induced collapse in Labradors (R298L), and a candidate mutation for epilepsy in humans (A447T) in vps1Δ cells to examine autophagic flux using again the Pho8Δ60 assay (Fig. 6A and Table 2).