To test the functional role of MPI in AML, we generated MPI KO and respective control non-targeting(NT)gRNA (hereafter Control) AML cells by CRISPR editing (Supplementary Fig. 2a) and confirmed that MPI KO sensitized FLT3ITD AML cells to FLT3-TKI (Fig. 2a). The gene discussed is FLT3; the disease is acute myeloid leukemia.