As a result, 19 genes, including CDCA5, CENPF, CENPW, CDCA8, SPC25, CDKN3, CENPA, BUB1, DLGAP5, IGSF3, HMMR, TOP2A, RAD54L, TTK, GINS1, PTTG1, ETV4, GINS2, and PKMYT1 were found to be independent prognostic biomarkers in HCC (Supplementary Table 8 and Supplementary Figure 3). The gene discussed is SPC25; the disease is hepatocellular carcinoma.