In a preliminary step, when we researched the correlation between TSEN54 and the expression of 20 m6A-related regulators through heatmaps on the basis of TCGA and ICGC databases in HCC, we detected that among them, 19 regulators revealed a significantly positive correlation with TSEN54 expression in each of TCGA and ICGC databases (P<0.05) (Figure 13A, 13B). This evidence concerns the gene TSEN54 and hepatocellular carcinoma.