To test if the paradigm also resulted in brain insulin resistance, we next analyzed the levels of phosphorylation of the insulin receptor substrate 1 (IRS1), an adapter protein of the insulin receptor with the capacity to either stimulate insulin signaling or to inhibit it depending on the phosphorylation state (Yaribeygi et al, 2019; James et al, 2021). Here, IRS1 is linked to Insulin resistance.