The amount of IRP2 was higher in WT HEK293T cells than in prime-edited HEK293T cells expressing endogenous NCOA4 C410A (Fig. 4, A and B), indicating that a defect in noncanonical ferritinophagy in WT cells might induce a response typical of cellular iron deficiency in normal culture medium conditions. Here, IREB2 is linked to Iron deficiency anemia.