In addition, western blot analyses showed that the expression of Tim-3, C/EBPβ, and Arg-1 were significantly decreased in the human decidual MDSCs after the infection with live T. gondii, whereas no alteration was found in the human decidual MDSCs treated with heat-killed T. gondii (Fig 7E), indicating that its effect may require the invasion of T. gondii. This evidence concerns the gene ARG1 and infection.