From the significantly decreased proteins in response to NSUN5 knockdown in U251 cells, we selected STAT3 and NSUN2 for validation by Western blotting and RT‐qPCR, because the protumorigenic functions of STAT3 in GBM has been well‐documented [41] and NSUN2 (another member of the NSUN family) has been shown to increase migration of U87 cells [42]. The gene discussed is NSUN5; the disease is glioblastoma.