The H&E staining, Masson staining, and the IHC staining showed that overexpression of SIRT1 could relieve liver fibrosis and reduce the expression of NF-κB P65, NLRP3, α-SMA, and iNOS in liver tissue of CCl4-induced liver fibrosis on Day 28 (Figure 2A-C, E-J). This evidence concerns the gene NOS2 and Hepatic fibrosis.