In addition, after cerebral ischemia reperfusion, acetylation of mitochondrial respiratory chain complex subunit 1 NDUFA9 was significantly upregulated, while NAD+ and NBP significantly decreased the acetylation of NDUFA9, suggesting that both NAD+ and NBP could facilitate the mitochondrial respiratory chain to improve mitochondrial function. The gene discussed is NDUFA9; the disease is brain ischemia.