To verify direct CXCL14 activation of NF-κB mediating EMT and migration, we pretreated lung cancer cells with the inhibitors of IκB kinase α (IKKα) and NF-κB inhibitor α (IκBα) or p65 siRNA to evaluate CXCL14-promoted cell migration and EMT. The gene discussed is CXCL14; the disease is lung carcinoma.