To dissect which signaling is regulated by FAM3C and involved in tumor EMT mediated by Edu-Neus, we found that FAM3C treatment up-regulated the expression of phosphorylated JNK (p-JNK) and EMT-associated transcription factors, ZEB1 and Snail, in MKN45 cells in a dose-dependent manner, but exerted no marked effects on expression of p-ERK, p-Akt, Slug and β-Catenin (Figure 3K, s4I). This evidence concerns the gene SNAI2 and neoplasm.