PRKACA and type 2 diabetes mellitus: HFHSD-induced type 2 diabetic mice were multi-point intramuscularly injected into the quadriceps of the two hind legs with lentivirus carrying- shRNA targeting PKACα (Lv-shPKACα) or control shRNA (Lv-shNC) and then these mice received 40 mg/kg TG treatment every two days for one month via an i.p. injection, which was the most effective therapeutic plan shown in our previous study (Fig. 3 and 11).