Likewise, TG administration, whether for prophylactic or therapeutic purposes, significantly upregulated the levels of p-AMPK in the skeletal muscle of T2DM mice in a dose-dependent manner, and p-AMPK levels in the muscles of T2DM mice administered with medium and high doses of TG were much higher than those of ND mice (Fig. 3E) Taken together, these results suggest that TG can activate AMPK signaling to enhance GLUT4 expression in muscles in vivo, which may mediate its anti-IR effects in HFHSD-induced T2DM mice. Here, SLC2A4 is linked to type 2 diabetes mellitus.