Finally, to determine whether the SIRT7/LAP2α axis plays a role in breast cancer metastasis in vivo, we injected SIRT7-knockdown, LAP2α-knockdown, or SIRT7-knockdown plus LAP2α-overexpression SK-BR-3 cells into the tail vein of athymic nude mice to achieve systemic dissemination, followed by tracking of metastatic colonization using a bioluminescent reporter. Here, SIRT7 is linked to breast cancer.