Interestingly, we found that the GC patients with a higher ratio of CD226+CD8+IFN-γ+TILs in CD8+CD226+TILs in the stromal region had poorer OS than those with a lower ratio (HR=1.976, 95% CI: 0.7865-4.966, P=0.0484, Figure 4D). The gene discussed is CD8A; the disease is gastric cancer.