As shown in Figure 5A, both 4-OHA treatment methods downregulated transcripts of ECM-related genes in DMBA-induce tumors, such as Col1a1, Tnc, Fn1, and Itga5. Meanwhile, the expression levels of these genes in MCF-7 and ZR-75-1 cells were downregulated by 4-OHA at a dose of 1 μM, which suggested that 4-OHA could cause an extracellular matrix remodeling of ER+ breast cancer cells. The gene discussed is COL1A1; the disease is breast cancer.