TNF and neoplasm: GSEA analysis of the three groups of CRC cells demonstrated that the tumor cell-intrinsic apoptotic signals “positive regulation of reactive oxygen species (ROS) biosynthetic process” and “p53 signaling pathway,” as well as the immune killing-related signals “type I IFN signaling pathway,” “MHC protein complex,” and “TNF signaling pathway,” were significantly enriched in CRC cells cocultured with IL-33-educated CD3+ T cells (Figure 6A-C).