The potential therapeutic benefit of using pharmacological inhibition of PANX1 channels by PBN or CBX has been previously reported in the context of human breast cancer in vivo17, 32, human melanoma cells in vitro and in chicken embryo xenografts 35, mouse testicular cancer cells in vitro55, and a mouse neuroblastoma cell line in vitro9. This evidence concerns the gene PANX1 and melanoma.