And EML4-ALK (E14:A21, allelic frequency: 14.05%) and SETD2-ALK (S12:A21, allelic frequency: 25.20%) double-fusion (Fig. 1) were found in the pleural tissue by DNA-based NGS, which can capture all exons of 31 genes that are highly related to the occurrence and development of cancer, and important exons and partial introns of 137 genes that are related to individualized treatment or high frequency mutation of lung cancer, and perform high-depth sequencing up to 1000X. Here, ALK is linked to lung cancer.