As shown in Fig. 4C, BUB1B was widely mutated across multiple cancer species, with the top 5 high-frequency mutations being Stomach and Esophageal carcinoma (STES), Uterine Corpus Endometrial Carcinoma (UCEC), Stomach adenocarcinoma (STAD), Esophageal carcinoma (ESCA), and Lung adenocarcinoma (LUAD) at 3.40%, 3.20%, 2.70%, 2.20%, and 1.70%. The gene discussed is BUB1B; the disease is carcinoma of esophagus.