Using a mouse model, where the RNA binding protein, serine-arginine-rich splicing factor 3 (SRSF3)27,28, is deleted in MK and platelets using Pf4-cre29 (Pf4-Srsf3Δ/Δ 30, herein termed MK-LCMΔ/Δ), normal MK numbers, but diminished LCM, elevated BM, PB and spleen HSC pools, as well as severe thrombocytopenia is observed. This evidence concerns the gene PF4 and Thrombocytopenia.