SW620 and HCT116 cells were cultured in the presence or absence of 10 μg/ml galectin-3, a concentration that is close to that seen in the circulation of colon cancer patients with metastasis [22] for 24 h, and the concentrations of proteases in the media was measured by the Proteome Profiler Human Protease Array which covers 35 common proteases. The gene discussed is LGALS3; the disease is colonic neoplasm.