Additionally, the luciferase reporter assay confirmed that POU6F2-AS2 directly targeted miR-125b-5p in NSCLC, as indicated by a marked decrease in luciferase activity in miR-125b-5p–overexpressing NSCLC cells cotransfected with wild-type (WT)-POU6F2-AS2 (Figure 4F). This evidence concerns the gene POU6F2 and non-small cell lung carcinoma.