POU6F2 and non-small cell lung carcinoma: To validate this observation, we performed real-time quantitative polymerase chain reaction (RT-qPCR) to determine the expression levels of POU6F2-AS2 in NSCLC tissues and the adjacent nontumorous tissues obtained from our cohort, which confirmed the upregulation of POU6F2-AS2 in NSCLC tissues (Figure 1C).