Comparison analysis revealed a significant increase in M2 macrophages (i.e., SELENOP+, SPP1+MARCO+, and SPP1+TGFBI+) and decrease in M1 macrophages (i.e., IL1B+, FCGBP+, and TXNIP+) in ATCs compared with PTCs (Figure 8B), suggesting that macrophages were reprogrammed from antitumor functions toward aggressiveness-promoting states. This evidence concerns the gene SPP1 and Ehlers-Danlos syndrome, musculocontractural type.