We separately validated the superior screening and diagnostic power of these markers with receiver operating characteristic (ROC) area under curve (AUC) (ROCAUC) analysis; the markers achieved a ROCAUC of 0.98 for distinguishing FH-mutant (FH-MT) RCC from nontumor normal control (NC) or FH–wild type (FH-WT) RCC samples. The gene discussed is FH; the disease is renal cell carcinoma.