CHD7 and CHARGE syndrome: Included in these were Sema3a and Sema3c (HCR staining with quantification in Supplementary material online, Figure S10), both genes reported to have decreased expression in later stage Mesp1 cKOs,13Chd7 heterozygous whole embryos,59 and, for Sema3a only, a Xenopus model of CHARGE syndrome.60 Notably, the Semaphorin3 receptor PlexinA2 is a BRG1:CHD7 target in NCCs.19 CHD7 has similar phenotypes in the NCC and mesoderm conditional mutants, in particular both have 4th PAA and OFT septation defects; mesodermal mutants have more severe AVSDs.