Notably, the expression of RhoA in ECs was upregulated when the monolayer was formed on the stiff (HCC) gel (Figure 2b), which is consistent with previous studies.[46, 47] Moreover, RhoA expression in ECs on the porous gel was significantly less than on the control substrate (p‐value = 0.0015, Figure 2b), implying that matrix porosity also affects EC‐ECM interactions and downstream mechanotransduction. Here, RHOA is linked to hepatocellular carcinoma.