To test whether YME1L-driven glioma cell growth was due to mediating Gαi1-Akt signaling, the recombinant adenovirus encoding the full-length Gαi1 (“Ad-Gαi1,” no tag) was stably transduced to koYME1L P1 glioma cells, that completely restored Gαi1 expression and Akt activation (Fig. 2H). Here, YME1L1 is linked to central nervous system cancer.