YME1L1 and central nervous system cancer: Fig. S4A confirmed that the YME1L mRNA expression was significantly elevated in OE-YME1L P1 glioma cells (versus vector control cells/“Vec”). In addition, significant YME1L protein elevation in total cell lysates was detected (Fig. S4B). YME1L protein upregulation was detected only in the mitochondria of P1 glioma cells with the YME1L-overexpressing lentiviral construct (Fig. S4B). Again no YME1L protein expression was detected in the mitochondria-null lysates (Fig. S4B).