YME1L1 and central nervous system cancer: As compared to control P1 glioma cells with the lentiviral scramble shRNA (shC) plus the CRSIPR/Cas9 empty vector (“lv-shC + Cas9-C”), YME1L mRNA expression was dramatically downregulated in shYME1L-expressing cells and koYME1L cells (Fig. 1J).