YME1L1 and glioma: In the YME1L-silenced or the koYME1L P1 glioma cells, the tetraethylbenzimidazolylcarbocyanine iodide (JC-1) color changed from orange (JC-1 aggregates) to green (JC-1 monomers) (Fig. S3A), indicating mitochondrial depolarization.