In other primary human glioma cells (P2 and P3) and immortalized cell lines (A172 and U251), lv-shYME1L-seq1-induced YME1L silencing similarly induced mitochondrial depolarization and ROS production, causing JC-1 green monomer accumulation (Fig. S3E) and the CellROX intensity increase (Fig. S3F). Here, YME1L1 is linked to central nervous system cancer.