YME1L1 and central nervous system cancer: In other primary human glioma cells (P2 and P3) and immortalized cell lines (A172 and U251), YME1L silencing by lv-shYME1L-seq1 (see Fig. 1) similarly induced caspase-3 activation (Fig. S2E) and cell apoptosis (evidenced by nuclear TUNEL ratio increase, Fig. S2F).