The previous analysis showed that Tregs cells are abundant in HCC tumors and are a subset of CD4+ T cells, a type of lymphocyte with high immunosuppressive properties.36 They suppress the immune response by inhibiting CD8+ T cell effector functions and directly promote tumor escape through a variety of contact-dependent and non-contact mechanisms.37 In HCC, neutrophils can recruit macrophages and Tregs into HCC by releasing cytokines, thereby promoting tumor progression and developing resistance to sorafenib.38 Here, CD8A is linked to hepatocellular carcinoma.