The top 5 DEmRNAs were Ryr3, Klf10, Dlg4, Osbpl5 and Hdac7. Among them, Ryr3 controls the intracellular Ca2+ levels and reproduces the core phenotypes of brain aging (i.e., neuroinflammation, neurotoxicity and cognitive deficits) (Liu et al., 2022). This evidence concerns the gene RYR3 and Cognitive impairment.