Considering that TNF-α and IFN-γ produced by immune cells can induce the secretion of pro-inflammatory cytokines for a strong anti-tumor immunity (23, 24), the levels of TNF-α and IFN-γ in primary and distant tumors were also detected on the 10th day after first treatment by ELISA to verify the activation of the tumor-cell killing of immune cells. This evidence concerns the gene IFNG and neoplasm.