However, the effects were completely or partially inhibited by adding ICI (P<0.05, P<0.01), which indicated that the three types of R. glutinosa may completely or partially regulate the TLR4/NF-κB/NLRP3 signaling pathway through the estrogen receptor pathway, thereby improving the LPS-induced inflammation in S-AKI mice and reducing kidney damage. This evidence concerns the gene ESR1 and Nephropathy.