TLR4 and food allergy: To investigate the role of calprotectin and inflammatory-related factors (TLR4, NF-κB, TNF-α, IL-6, and IL-1β) in food allergy, Zhu et al. [57] selected 80 3-week-old male Brown Norway rats: 40 rats were randomly assigned to the OVA-sensitized experimental group, while 40 rats were assigned to the normal saline sham-sensitized control group.