To verify whether EREG was a target gene of miR-330-3p in BC, through the dual-luciferase reporter assay, we found that miR-330-3p mimic remarkably decreased the relative luciferase activity of the WT EREG (32.7%, p = 0.002) in MCF-7 cells but did not affect that of the MUT EREG (Figure 6A). Here, EREG is linked to breast cancer.