However, among the 20 most affected signalling pathways, the NF-kappa-B inhibitor cactus (Cactus) gene is of particular interest because of its involvement in several different signalling pathways, including the toxoplasmosis, pathogenic Escherichia coli infection, insulin resistance, and Toll and IMD pathway (Table 6). Here, TLR4 is linked to toxoplasmosis.