Compared with the Con-group, 62.5 μg/mL, 125 μg/mL, 250 μg/mL, and 500 μg/mL TB increased the TLR2 expression by 1.08, 1.45, 1.46, and 1.45 times and the TLR4 expression by 1.42, 1.97, 2.36, and 2.06 times, respectively, indicating that TB can activate TLR2/4 at the same time. This evidence concerns the gene TLR2 and tuberculosis.