To eliminate the effect of autofluorescence, which can be expressed in yolk sac lipids [31] (see Figure 1D, lateral view of yolk sac at 1 dpi), we calculated corresponding corrected total cell fluorescence (CTCF) values, a technique used to subtract background fluorescence in zebrafish cancer models [32], and found that the area occupied by TNBC cells increased over the 1, 4, 6, and 8 dpi time points (mean CTCF values in relative fluorescence units (RFU): 110.25 (1 dpi), 408.99 (4 dpi), 553.55 (6 dpi), 734.01 (8 dpi); Figure 1E). This evidence concerns the gene CTCF and cancer.