Recurrent RSPO2 mutations have also been identified in HCC as a result of a large deletion of chromosome 8q23.1, and whilst the authors speculate a similar EIF3E-RPSO2 mutation to that found in CRC, they were unable to verify an EIF3E-RSPO2 amplicon due to DNA and RNA fragmentation in the formalin-fixed paraffin-embedded samples used [53]. This evidence concerns the gene RSPO2 and hepatocellular carcinoma.