The twenty-six miRNAs down-regulated during FU in PCOS patients in total target mRNAs were significantly enriched in pathways such as membrane trafficking, nervous system development, axon guidance, GnRH receptor pathways, and post-translational modification (Figure 5), suggesting that target gene depression could occur during FU to allow for increased GnRH signaling and, possibly, improved feedback from gonadal steroids [54]. Here, GNRH1 is linked to polycystic ovary syndrome.